欧美边做饭边被躁bd在线看,欧美激情一区二区三区,亚洲熟妇av一区二区三区宅男,国产av在线观看

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1772C2C12 小鼠成肌細(xì)胞

C2C12 小鼠成肌細(xì)胞

簡(jiǎn)要描述:CRL-1772 C2C12 小鼠成肌細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-26
  • 訪(fǎng)  問(wèn)  量:2384

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞的詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™  Price:$256.00
Designations:C2C12

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:myoblast
C2C12 小鼠成肌細(xì)胞


Source:Strain: C3H
Tissue: muscle
Cell Type: myoblast;


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this . AATCC materialnyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1772

Applications:transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)



Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903]

The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953]

Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427]

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.

Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.

Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

    Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.

  6. Incubate cultures at 37°C.





















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
麻豆国产一区二区三区四区| 精品国产污污免费网站入口| 色综合久久一区二区三区| 少妇aaa级久久久无码精品片| 久久aⅴ无码av高潮av喷吹| 小婷又嫩又紧又滑又多水| 公侵犯玩弄漂亮人妻优| 亚洲阿v天堂在线| 久久精品人妻一区二区三区| 亚洲av永久无码精品古装片| 色多多性虎精品无码av| 亚洲乱亚洲乱少妇无码99P| 国产97在线 | 免费| 国产偷亚洲偷欧美偷精品| 午夜无码人妻av大片色欲| 国产偷窥熟妇高潮呻吟| 宝宝好涨水快流出来免费视频| 永久免费不卡在线观看黄网站 | 日本三级欧美三级人妇视频| 亚洲午夜精品A片久久| 《超大爆乳护士》在线观看| 老色69久久九九精品高潮| 无码熟熟妇丰满人妻啪啪软件| 97久久精品人人澡人人爽| 国产vpswindows精品| 强开小婷嫩苞又嫩又紧视频韩国| 熟妇人妻久久中文字幕| 免费无遮挡无码永久视频| 精品免费囯产一区二区三区四区| 337p粉嫩日本欧洲亚洲福利| 亚洲av午夜精品一区二区三区| 日本丰满少妇xxxx| 国产真实偷人视频| 久久久97精品国产一区蜜桃| 久久久精品波多野结衣AV| 国产熟女乱子视频正在播放| 国产精品无码专区av在线播放| 99草草国产熟女视频在线| 无码人妻一区二区三区在线| 凡人修仙传动漫在线观看| 高清freexxxx性国产|